Yuhong Mao

5 Questions 6 Answers 0 Followers

Questions related from Yuhong Mao

Protein size measurement using Zetasizer?

The idea is to measure the hydro- protein sizes in various solvents (eg. with high ionic strength, EtOH/Water, other organic/water solvents), then, due to the extremely low solubility of some...

29 January 2021 440 3 View

Fitting with a Piecewise Linear Function in origin?

Hello, I'm using the Piecewise Linear Function in origin to fit my data, which should contain 9 segments. According to the method from...

29 July 2016 3,027 4 View

Is Michaelis-Menten theory suitable for IMERs?

Michaelis-Menten theory is valid only for a catalytic amount of enzymes and only apparent parameters can be obtained for IMERs. Others approaches could be considered (ex. frontal analysis). Why?

19 November 2014 3,503 3 View

Protein structure in various solvents — Chiral dichroism

Dear all, I want to know the structural changes of a protein in various solvents using CD technique. Then, questions come: (1) Buffer baselines: I would like to check the structures of my protein...

01 January 1970 5,888 3 View

Covalently bonded enzyme showed worse thermal stability than its free form?

we immobilized enzymes on epoxy or amino resins, and just find decreased thermal stbaility (compared with their free forms). We are wondering whether the covalent bonds are formed. However, we did...

01 January 1970 8,154 4 View