12 Questions 17 Answers 0 Followers
Questions related from Yuhong Mao
Hello all, I'm using Discovery studio 2019 to dock protein-protein, while "An error was encountered during execution of ViewResults.ds_pl.", then the dock failed. As it works quite well with the...
01 December 2022 896 4 View
The idea is to measure the hydro- protein sizes in various solvents (eg. with high ionic strength, EtOH/Water, other organic/water solvents), then, due to the extremely low solubility of some...
29 January 2021 566 3 View
I just installed the software FlexAnalysis 3.3, when I tried to analyse the mass spectrum, firstly have to do the calibration. Normally there is a Mass control list to chose the...
04 November 2016 2,233 3 View
Hello, I'm using the Piecewise Linear Function in origin to fit my data, which should contain 9 segments. According to the method from...
29 July 2016 3,100 4 View
According to pH stat method, the hydrolysis degree of my samples always lower than 5%, for these samples, is it suitable to use OPA method? I tried, but for some hydrolysates, the absorbance is...
26 February 2016 7,292 5 View
Hello all, I measured protein contents before and after hydrolysis, but the result is very weird...... The initial protein concentration was around 2 mg/ml, and the limited concentration for my...
16 June 2015 5,562 9 View
Michaelis-Menten theory is valid only for a catalytic amount of enzymes and only apparent parameters can be obtained for IMERs. Others approaches could be considered (ex. frontal analysis). Why?
19 November 2014 3,550 3 View
Dear all, I want to know the structural changes of a protein in various solvents using CD technique. Then, questions come: (1) Buffer baselines: I would like to check the structures of my protein...
01 January 1970 5,955 3 View
we immobilized enzymes on epoxy or amino resins, and just find decreased thermal stbaility (compared with their free forms). We are wondering whether the covalent bonds are formed. However, we did...
01 January 1970 8,209 4 View
I explored NaCl with different concentrations (0, 0.02, 0.1, and 0.5 M) on the beta-Lg hydrolysis in a flow-through monolithic reactor with immobilized trypsin. The hydrolysis efficiency was...
01 January 1970 1,287 0 View
Hello all, consedring the mechanism of enzymatic hydrolysis, such as proteases - peptide bonds, the first step is always the contact between them, then one question comes to mind: is it true that...
01 January 1970 5,399 6 View
Hello, I am working with Bruker Tensor 27, while before measuring a sample, the automatic tests for OQ and PQ are always failed. Then, I think that the obtained data is not reliable, right? So...
01 January 1970 5,182 0 View
