Protein quantification using GFP

18 May 2017 1 6K Report

Hello, I am currently working on GPCRs and am about to create multiple mutants to see their effects on expression level. They are tagged with GFP but am I able to quantify exactly how much receptors I have using just GFP, as radioligand binding is not available.

Huanting Liu

you can quantify the expressed receptors using quantitative GFP fluorescence similar to that described for soluble protein on https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3315830/.

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