02 February 2017 6 8K Report

Hi,

I am trying to purify the chimeric fusion proteins containing NGF and various functional domains of Fibronectin. Although lower molecular weight proteins were secreted into the conditioned media (up to 70 KDa) and successfully purified, higher mwt proteins were not secreted (>75 KDa, 104 KDa and 132 KDa).

My question

1) What could be the potential reasons that not secreted

2) Which procedure can be useful to purify (6X-His-tag) these proteins

Thank you

Raju

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