Raju Tippana

6 Questions 15 Answers 0 Followers

Questions related from Raju Tippana

Protein purification from Sf9 insect cells

Hi, I am trying to purify the chimeric fusion proteins containing NGF and various functional domains of Fibronectin. Although lower molecular weight proteins were secreted into the conditioned...

02 February 2017 8,064 6 View

NGF Characterization assays on PC12 Cells?

can any one suggest what potential experiments could be performed to test the NGF activity on PC12 cells except Neurite outgrowth, migration & proliferation.

04 April 2016 9,165 3 View

Any suggestions on the cell lysis buffer for his tag protein purification?

Hi Researchers, Following to my previous questions, few of my chimeras are unable to secrete into media as expected but present in cells. Current plan is to purify from cells and I tried once with...

05 May 2015 5,087 4 View

It is mandatory to remove imidazole after recom protein purification for cell based functional assays?

Hello researchers, I'm working on chimeric fusion proteins (NGF:FN) growth factors and ECM protein combination. I have expressed this protein in insect cell system and purified on Cobalt resin...

03 March 2015 1,991 13 View

Does anyone have set hands in purifying the his tagged recom protein (secretory) NGF?

I'm working on Novel protein therapeutics for peripheral nerve regeneration. My protein NGF (Nerve Growth Factor) was expressed in Sf9 cells and secretory protein was unable to purify under native...

10 October 2014 342 4 View

Does any of you have protocol for insect cell (Sf9) expression protocol?

I'm doing my phd on peripheral nerve regeneration. My vector pib/v5-his.

01 January 2014 6,724 2 View