Poushali Bose

2 Questions 1 Answers 0 Followers

Questions related from Poushali Bose

Protein purification for his tagged protein?

pI of my protein is 4.1. I am using Ni coumn to purify it . Which binding buffers will be good? Even the pI is low can it still bind to nucleic acid? How do I know?

05 May 2024 8,429 2 View

I am purifying a protein remaining in bound condition to RNA and I want to analyze both of them separately. Can anyone suggest some ideas please??

I tried to use RNAase to study protein and I have tried to digest protein to get nucleic acid also. The problem is yield is low and RNA is fragmented. I am trying to purify both of them in...

18 January 2024 4,859 3 View