What should be the optimum protein in micrograms to be loaded per well for better blotting in the mini 1.5 mm thick sds gel well which has 7 wells of 6mm width
I am operating a continuous cultivation at fixed carbon and nitrogen concentrations, and under carbon-limitation regime and varying dilution rates from 0.1 to 0.01/hr. Under these conditions all...
13 May 2024 2,510 0 View
Restriction enzyme that i used for double digestion is EcoR1 and Xho1 for 1,2 and 3 hours but didnt get digested band on gel. RE is of Genei company.
01 May 2024 3,580 1 View
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09 April 2024 7,398 1 View
Productivity in case of filamentous fungi is heavily depend upon the morphology of the fungi. But during submerged fermentation, morphology is function of impeller design, speed and orientation of...
15 October 2023 4,123 9 View
in nitrification process nitrate will form later denitrification will happen normally. but some research article they used anoxic first later they used aerobic
18 September 2023 665 0 View
Hi, could someone tell me whether I can autoclave Glucose -6-phosphate or should opt for filter sterilization?
25 August 2023 1,704 2 View
Since NOx is highly soluble in water, what is the method for detecting the precise quantity of NOx generated in the system? For example, let us consider the system of oxy-hydrogen combustion where...
25 May 2023 2,934 3 View
I am preparing sulfonic acids from alkyl bromides using sodium sulfite as sulfonation reagent, along with my desired product NaBr is forming, The desired compound and NaBr has good solubility in...
30 March 2022 1,521 1 View
Currently I'm working on a project using RFID technology. As a part of that I need to attach the RFID IC (NXP UCODE G2iL IC) to the antenna. Anyone help me on how to attach the IC like which pins...
21 November 2021 1,507 7 View
I am working on Trichoderma atroviride aromatic compound production. Earlier, I observed the spore as well as mycelial growth on the plate but now I am getting only spores on the plate without...
24 October 2021 2,415 13 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View