I read an article on the topic protein extraction from bacterial suspension . It was written that "residual of acetone was removed under the stream of nitrogen"
How can I carry out the same in the lab?What is acctually meant by stream of nitrogen?
it just means blowing nitrogen gas gently over the wet residue of precipitated protein until all of the liquid has evaporated and the protein is dried. Cylinder stored nitrogen is very dry and pure and the drying process is slightly quicker and cleaner than blowing air over the sample. If you just leave your sample to evaporate until it is dry you will gwt the same result but it will take a little longer
In many cases, a steady stream of gas is used to evaporate the solvent. Pure, dry nitrogen is commonly used to evaporate solvents, as it is a relatively non-reactive gas; in contrast, air contains oxygen and water vapor, which could react with the compounds of interest.
https://www.youtube.com/watch?v=bOpHvch0vQg
Agreed with @Paul Rutland. blowing nitrogen gas allows you to get your sample dried quickly.
Hi,
There is also speedvac vacuum concentrator option as an alternative for concentrating and solvent removal. But if you have doubt to use nitrogen and/or speedvac than you can use spin columns, desalting cartridges, SEC columns, or ultrafiltration devices and performing several washing to get rid of residual acetone in place of the aforementioned powerful techniques.
Good Luck
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