Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Analysis of CRD two factor using R studio ?

Hello everyone. I have conducted the research on post harvest longevity of tomato crop using plastic packaging and different doses of chemicals as two different factors. The design of experiment I...

28 February 2021 8,189 3 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

How can I selectively dry etch Ti on a polymer substrate?

24 February 2021 5,378 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

What are your alternative data collection methods during the pandemic? What are the pros and cons?

Dear colleagues We are exploring possible data collection methods for environment and behavior research in the context of architectural environments. Have you conducted any Post Occupancy...

16 February 2021 2,575 4 View

Can bio-decomposer be a successful alternative to reduce residue burning?

To maintain the proper crop plan most of the times there is not enough time to make the field ready for the next crop. In this particular context , can bio-decompser be a solution? What about...

16 February 2021 7,068 24 View

Is there any effective FEM solver to perform simulation of balloon inflation with large deformation (e.g. 300% strain)?

Hi! Thank you for your time, attention, and support. I am currently using FEM solver Abaqus to perform a set of balloon inflation simulations, trying to understand the deformation shape, stress,...

14 February 2021 2,524 4 View

Unable to detect a truncated protein variant, but I can observe a truncated cDNA from Sanger?

Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...

17 February 2021 1,130 6 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Using the Taqman PCR, how does one know which allele is being determined by VIC / FAM?

I am exploring a number of SNPs, unfortunately although the test are ok, I am perplexed because my preliminary results are nearly all against what is reported in the literature. To confirm my...

29 January 2021 3,905 2 View

Problems with allele speration by cloning in pKS plasmid?

I am trying to do the allelic separation of a ~8 kb region using cloning strategy with pKS plasmid. However, when I analyze the result of the colony PCR sequencing It seems that both alleles were...

14 December 2020 5,829 2 View

Weak SSR peak on capillary electrophoresis trace - is it a heterozygote?

Looking at the attached image, the top two samples show a weak peak at 212 compared to the very strong 200 peak. The bottom samples is homozygous for the 212 peak. Since the samples look a bit...

27 October 2020 2,353 3 View

How can I differentiate the true allele from stutters and PCR artifacts in microsatellite genotyping?

Most of my microsatellite markers are dinucleotides, therefore they show stuttering. In regular stuttering is easy to identify the allele, which is the highest peak on the right, after the shorter...

26 September 2020 1,486 5 View

Hi for every one I need the primer for ABO rs495828 ( ARMS or allele specific PCR)?

the primer for PCR ( ARMS or allele-specific ) for scientific research either from the studies or someone who has the ability to deisgn the primer for me as a required features above/ thank a lot...

20 September 2020 224 4 View

Is there any way to design allele specific PCR primers for a single nucleotide "deletion"?

Hi, I am currently doing a research on Inborn errors of metabolism in Sri Lanka and for that, I need to develop a method to identify few SNPs and a single point deletion using allele specific PCR...

27 August 2020 2,411 4 View

Does anyone know how can I design primer for hla_DRB1*13:03 allele?

I would like to investigate association between this allele and a disease, but sequence of this allele is very similar with other alleles in hla_drb1 alleles group, I don't know how can I design...

18 August 2020 6,560 2 View

How to test if two SNPs within a gene are on separate alleles?

Hi everyone, I have a question regarding sequencing SNPs. I have currently creating two SNPs on one gene and these SNPs are about 8kb apart from each other on two different exons. I would like to...

04 August 2020 5,531 3 View