Hi everyone, and thanks in advance for reading!
I want to determine oxidative stress human serum/plasma using protein damage; I´ve seen there are a lot of kits and methods, I want to try an ELISA for this, but as I read more and more papers, the methods described are many times vague and unclear.
Please, If someone is doing this in his Lab or have experience on this issue, can you please reach me a protocol for sample preparation and protein carbonylation detection?
It will be very useful as I see there are a lot of steps and I will save a lot of time (and money) on starting it up making my own mess...
Thank you all!