How do you propagate freeze-dried F.nucleatum from ATCC? Is it OK to add medium to the vial under aerobic condition and transfer the tube to an anaerobic chamber for further growth?
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Is it possible to tell Proturan families apart based on overall appearance? All the keys I have seen are based on very fine characteristics only readily visible on a slide. Also, there seem to be...
14 June 2024 6,334 0 View
Hello :) I am looking at the presence of toxin biosythensis genes in lake sediments. Nothing fancy, since I only want to verify the specificity of PCR results for primers related to marker genes...
27 May 2024 392 4 View
Hello, Since a large part of my budget will go on NGS and I don't have the money to repeat it, I want to make sure that it will work. The PCR products look good on the gel (nice bands of the...
21 April 2024 7,785 3 View
So I know we should round the value of R Square to two decimal points, but how should you do it if the value is .001. Should you report it as R2 = .00, or R2 = .01?
26 March 2024 1,387 2 View
Has anyone run slide in Visium of tissue sections (Heart, kidney, brain) with CMC than OCT for spatial transcriptomics ???
14 March 2024 243 2 View
I aim to measure the concentration of hydrocarbon based organic compound from a certain quantity of soil sample. I would really appreciate it if anyone can give me some experimental guidelines.
07 January 2024 2,549 1 View
I've requested access to "Black People under the Black Flag - Arne Bialuschewski" and since this is my first time using this program i wanted to ask, how long it normally takes to get access to a...
14 December 2023 9,128 1 View
Hello, I conducted mouse tumor digestion using two different protocols: one involving 0.1% trypsinization and the other using 0.4 mg/ml collagenase + 1.25 U/ml dispase II. The chopped tumor was...
22 November 2023 8,055 0 View
The density of the quartz sand and non-magnetic particles are nearly the same, I already tried to separate it in water as a first try, but all particles sunk down. There could be a difference in...
03 November 2023 2,371 3 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi researchers! I'm working on soil texture analysis, and the end result for sand is doubtful because there is black sediment appearing after drying, as shown in the figure. Is it considered sand?...
30 July 2024 557 2 View
My screen printed electrode CV is given here. For Nerstean one electron reaction the delta E value should be close to 57 mV and ration of Ic/Ia should be close to 1. I did CV of my screen printed...
30 July 2024 4,671 3 View
I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.
29 July 2024 6,115 0 View
Hey researchers! I am currently doing a research about to what extent in which A has accelerated the inclusiveness of the payment system in my country. Down below are a few examples of the...
26 July 2024 5,654 3 View
I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....
24 July 2024 4,058 5 View
Machine Learning
24 July 2024 2,487 3 View
Hi everyone, Can someone explain for me a method to inject VOC collected with Tedlar bag from pyrolysis process and transfer or inject into GCMS. Thanks
22 July 2024 6,398 1 View
Hello, I have a somewhat weird question: has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO? Have you managed to correct it? There is some...
19 July 2024 3,094 2 View
Dear Participants- Faculty and Researchers from Pakistani Higher Education Institutions, Thank you for participating in research study that aims to 'Analyze Individual and Institutional Factors...
18 July 2024 7,024 1 View