Where can I get LN-308 cell line?

Hi! I am looking for a cancer cell line with endogenously high expression of GPR55. LN-308 seems to have the highest level of GPR55 among multiple cancer cell lines (according to Genevestigator;...

20 October 2020 3,004 3 View

How can I quantify bacteria viable cells in a non-sterilized organic compost?

I'm working with the enrichment of an organic compost with PGPR, but this compost is not sterilized. How could I quantify viable cells of Azospirillum brasilense, Bacillus subtilis, and...

01 April 2020 9,486 4 View

How to model near-stall compressor operation with RANS CFD?

Hello everyone. I am looking into near-stall behaviour of the multistage axial compressor using CFD with RANS method. What is the best practice to establish if the compressor is close to stall,...

09 March 2020 8,847 8 View

What iron species are precipitating after autoclaving ferric chloride water solution?

I made 10mM FeCl3 solution in MQ water. After autoclaving it (standard 15min procedure) solution turns orange-brown. Filtering using 0.22um filter does not remove the color (but some of it is...

24 September 2019 2,407 7 View

What is a typical RFU value for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer?

Hi, quick question, What typical RFU value should I expect for a "low concentration" standard sample when quantifying RNA with Quantus Fluorometer? I made two standards (100 uL dye buffer, 98 uL...

10 April 2018 5,090 3 View

RNAprotect reagent formulation?

Hello, For the project I am currently involved in, I am in need for a cheap, non-toxic, pH 7+, non-disruptive buffer for RNA stabilization of bacterial cells (applied to a large environmental...

04 March 2018 5,089 14 View

How much does ammonium sulfate precipitation affect nucleic acid integrity?

Hello, In my current project I am at present trying to tackle an issue of rapidly stabilizing bacterial RNA in a large environmental sample. Most known stabilization reagents are not applicable in...

04 March 2018 1,589 3 View

SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data and Light Pollution Assessment

We are working with SUOMI-NPP VIIRS DNB (Day-Night Band) radiance data, which has units of W cm-2 sr-1. The case study area is a bird sanctuary and an inhabited territory that is also used for...

14 May 2017 7,715 2 View

Is there a relation between Hadamard product and standard matrix product?

05 July 2015 5,366 7 View

Can we get the sufficient number of intensity measurements for a complex vector reconstruction in quantum tomography?

In a paper arXiv:1312.0158 the authors proved that any complex vector (thus also the quantum state vector) can be uniquely determined (up to a global phase factor) by 4N-4 generic intensity...

22 June 2015 7,125 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View