Hi. I have recently started a culture of MDA-MD-231 from ATCC. However, the culture conditions by ATCC require culturing in an incubator without CO2 supplementation. But we only have CO2 incubators. I also see that many people culture it in DMEM with 10% FBS. I am culturing mine with 10% High Glucose DMEM supplemented with L-glutamine from Hyclone. However, my culture takes forever to become confluent, and there is always many dead cells even if I change the medium every 2 days.
May I know if anyone experience this and if there is a solution?
- Badges
- Science topic
More Desmond Wong's questions See All
Similar questions and discussions