Desmond Wong

2 Questions 4 Answers 0 Followers

Questions related from Desmond Wong

Problem with culturing MDA-MD-231 in DMEM?

Hi. I have recently started a culture of MDA-MD-231 from ATCC. However, the culture conditions by ATCC require culturing in an incubator without CO2 supplementation. But we only have CO2...

21 June 2022 4,956 0 View

How to quantitate protein in conditioned supernatant?

I am trying to purify some secreted protein in culture medium containing 10% FBS. But I need to know what is the amount of protein in the medium to do this. I will be using the Bradford Assay for...

14 March 2022 2,221 8 View