Hello! Can anyone help me please? I have sent purified bacterial sample of a bioluminescent bacteria isolated from a squid's eye to a commercial sequencing lab. The results came today, but the sequence similarity is showing 91% homology with Pseudomonas aeruginosa. The problem is we did morphological and biochemical tests in the lab on this bacterium and it does not look at all like Pseudomonas aeruginosa I don't know what went wrong. It is Gram-negative, pinpoint and extremely bioluminescent bacteria. I am confused. Is it a new bacterium or there is a technical mistake??
Given the 91% homology to P. aeruginosa you observed, have you also compared to other close species like P. fluorescence or P. syringea? These are also capable of bioluminiscence and may be closer matches to your isolate. So. Probably querying for and clustering these 3 species together with your sequences may give you a better idea of what you have.
Thanks very much. I have checked with the company. It was a mixed template. So I am sending the sample again. I guess it got contaminated during transport.
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