This is very good method to prevent gel leakage when casting the gel, but I found out that when running the sample in gel. The sample can only go down max and stuck at around 1cm above the high temed gel. If I keep running the low MW protein will become smile shape.
The way I done is I add a small volume (300-400uL, lower than this may not cover the whole bottom) of resolving gel with high concentration of temed into gel glasses first, to seal the bottom.
Have you guys had experience using this prevent gel method? How do you normally deal with/solve this problem? I would like the sample could go a little bit lower to detect different molecular weight protein signals on the same gel for western blot. Increase %gel could make my protein targets to close to each other.
Thank you
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