I make RNA extraction from Ambrosia plant leaves with triozol methode, the problem that in the end i have low yield and bad absorbance at 260 is too low (less than 1 and it supposed to be between 1.8 and 23 ) ,from my reading found that the resone may be due to high penol content of the tissue , although i wash 3 times with choloroform and 3 time with ethanol .
why this happend and how to resolve it ?? neeed help
Aparna Gunda
for low yield- may be they are loosing RNA duirng the protocol. make sure that you finish it as fast as possble, within 1 hour.
make sure all the reagents, pipettes, work bench and centrifuges are thoroughly cleaned and plastic ware double autoclaved, useDEPC water or Rnase free water, available commercially
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