Hi,

Quick conceptual question; in any assay that relies on enriching DNA with an oligo probe, e.g. having a biotinylated oligo that can be pulled down with streptavidin coated beads; How would one limit re-hybridization of the target DNA strand with the complementary DNA strand instead of with the oligo? Of course one could have a mostly ssDNA input (e.g. through asymmetric PCR) but how could this work if the input is a (originally double stranded) DNA that was simply denatured? Wouldn't the (presumably) longer complementary DNA strand always be more likely to rehybridize with the target DNA than the presumably shorter oligo, even if the oligo is present in excess?