4 Questions 2 Answers 0 Followers
Questions related from Sigrun S.
Hi everyone, I am trying to knock out a non-coding region downstream of my gene of interest. I looked at a publication which did exactly this but in a different species. They seemed to use two...
25 June 2022 9,524 3 View
Hi everyone, I'm looking for an alternative to sonication or benzonase for lysing DNA in Western Blot samples. Briefly, I am performing a subcellular fractionation on 3 Mio cells with a...
18 October 2017 9,517 2 View
Hi guys, I am hoping to get some advice / insights into something I'm seeing in the lab. So I'm activating Jurkats with anti-CD3 and anti-CD28 antibodies for 0-10 minutes. After cell lysis I...
07 November 2016 6,290 2 View
Hi everyone, We have some liquid cultures growing that we would like to use for both making long-term glyercol stocks and for purifying plasmids from them. In between the liquid culture being...
22 June 2016 9,323 4 View