Primers forward and reverse for the molecular marker named MahSe2 ?

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Abhijeet Roy

If you have a sequence of this marker then you can design a primer pair using NCBI Primer-BLAST (https://www.ncbi.nlm.nih.gov/tools/primer-blast/). Here is the article link: Article Primer-BLAST: a tool to design target-specific primers for p...

Moustapha Boubacar

I dont have this sequence. It is my request object. I know only the name of this marker. Thanks for your answer.

Ali Raza

Hey Moustapha Boubacar

MahSe2, recently identified as 61R-M2 . See page 148 for your reference

https://s3.amazonaws.com/academia.edu.documents/51490412/Cowpea20170123-384-sx910r.pdf?response-content-disposition=inline%3B%20filename%3DCowpea.pdf&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Credential=AKIAIWOWYYGZ2Y53UL3A%2F20200109%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Date=20200109T010324Z&X-Amz-Expires=3600&X-Amz-SignedHeaders=host&X-Amz-Signature=fa9eebce41ac9212c6141e56ca580c3a7fa9a3688d63541bbcb95930e0ad118d

and here you can find the sequence of this primer.

Article Breeding cowpea for resistance to Striga gesnerioides in the...

Regards

Ali

The MahSe2 marker is different of C42-2B. Do you mean they are the same? I have'nt access to the website that you tell me.

Thanks for your answer

Best regards