Primers for qPCR on CRISPR gRNAs?

04 April 2018 1 7K Report

Due to short sequence length and strong secondary structure I find difficult to target CRISPR gRNAs by qPCR. The sequence of the gRNA I am targeting is 5´-(N20)-GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCCGTTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTTTTTT-3´.

Has anyone successfully qPCRed such gRNAs? Could you share the primer sequences with me?

Thanks.

Hassan Nima

this link is useful

https://www.biocat.com/genomics/genome.../crispr-cas9-rt-pcr-prime...

regards

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