In our lab we investigate the biofilm formation of uropathogenic E. coli and I would like to perform a random mutagenesis experiment to be able to identify genes mediating this process.
However, I lack any suitable screening assay to select those mutants with defective biofilm formation. Because I am actually pursuing a negative phenotype (the lack of biofilm formation) I find it hard to conceive a suitable screening for this case.
Could anybody suggest an elegant screening method to identify biofilm-defective mutants?
I would recommend you to read articles of Michael J. Franklin from Montana State University, USA. He and his co-authors have plenty publications related to this topic. Besides, read articles of Roberto Cotler, a guru on this topic. Good luck. IB
Dear all,
Thank you for your suggestions, I will go through their papers and collect ideas.
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