Dear Babita,
There are a number of methods that have been published for HIV drug resistance. Please find below a sample of 5 papers which included primers. All of them have been validated on subtype C samples (including one from India).
1: Acharya A, Vaniawala S, Shah P, Misra RN, Wani M, Mukhopadhyaya PN.
Development, validation and clinical evaluation of a low cost in-house HIV-1 drug
resistance genotyping assay for Indian patients. PLoS One. 2014 Aug
26;9(8):e105790. doi: 10.1371/journal.pone.0105790. PubMed PMID: 25157501; PubMed
Central PMCID: PMC4144911.
2: Manasa J, Danaviah S, Pillay S, Padayachee P, Mthiyane H, Mkhize C, Lessells
RJ, Seebregts C, de Wit TF, Viljoen J, Katzenstein D, De Oliveira T. An
affordable HIV-1 drug resistance monitoring method for resource limited settings.
J Vis Exp. 2014 Mar 30;(85). doi: 10.3791/51242. PubMed PMID: 24747156; PubMed
Central PMCID: PMC4024245.
3: Chaturbhuj DN, Nirmalkar AP, Paranjape RS, Tripathy SP. Evaluation of a cost
effective in-house method for HIV-1 drug resistance genotyping using plasma
samples. PLoS One. 2014 Feb 12;9(2):e87441. doi: 10.1371/journal.pone.0087441.
PubMed PMID: 24533056; PubMed Central PMCID: PMC3922725.
4: Bronze M, Aitken SC, Wallis CL, Steegen K, Stuyver LJ, de Wit TF, Stevens W.
Evaluation of an affordable HIV-1 virological failure assay and antiretroviral
drug resistance genotyping protocol. J Virol Methods. 2013 Dec;194(1-2):300-7.
doi: 10.1016/j.jviromet.2013.08.015. PubMed PMID: 23994150.
5: Zhou Z, Wagar N, DeVos JR, Rottinghaus E, Diallo K, Nguyen DB, Bassey O,
Ugbena R, Wadonda-Kabondo N, McConnell MS, Zulu I, Chilima B, Nkengasong J, Yang
C. Optimization of a low cost and broadly sensitive genotyping assay for HIV-1
drug resistance surveillance and monitoring in resource-limited settings. PLoS
One. 2011;6(11):e28184. doi: 10.1371/journal.pone.0028184. PubMed PMID: 22132237;
PubMed Central PMCID: PMC3223235.
You can review these papers and select the primers for your genotyping.
I hope the above information will be helpful. Please do not hesitate to contact me if you need any further help.
Best regards,
Justen
I am not an expert, but you may consult this database (one of the best in the world for HIV drug resistance).
https://rega.kuleuven.be/cev/regadb
Dear Justen Manasa
Thanks for your kind words and support.
Hope with this I can proceed.
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