Hi. I am diving in the new work of primary cell culture. I am culturing primary neurons from dissociated cells (Rat E18). My main purpose is to grow them, have them form a network and measure their activity (electrophys/imaging).
I know that astroglia co culture is not necessary but my questions are:
1. What is the difference (physiolo/electrphys) for primary culture of neuron with and without astroglia support
2. I am using a protocol that perform direct co culture astroglia/neuron (not astroglia first then neurons). Has any one does it successfully ?
Thank you for your help
I am using dissociated cells from the same tissue. I work with brainbit solution and they have a co culture media which you switch when astroglia reach confluence.
Nicholas points to a great aspect of co culture, the fact that synaptic release is more local might help the network to be less tonically excited which could reduce overall response. This might explain why conculture are more active and responsive.
thank you!
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