Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

01 December 2020 8,792 3 View

Advices for: no amplification of aimed gene from transfected cells seleceted for it?

From a population of transfected/transduced cells I sort those with the desired expression. Starting from 10.000 cells I extract the ARN, and from its cDNA I amplify the gene of interest, as well...

19 September 2020 8,378 3 View

Measure of intracellular lactate?

Hi! I will try to measure intracellular lactate through lysing the cells in 20 mM Tris-HCl, 150 mM NaCl and 1% NP40. I'm going to use a clinical kit, which is constituted of Pipes buffer, pH 6.8....

30 January 2020 5,211 2 View

Dissolved oxygena DYE for cell culture?

Hi, I am looking for a dye to monitor O2 changes in cell culture media. I would like to have it colorimetric and not fluorescent but since I am not finding anything I could to fluorescent too! I...

23 October 2018 5,895 2 View

How do I determine the sample size to evaluate the performance of an image segmentation algorithm?

I have three image segmentation techniques that I have developed. I wish to compare the performance of the segmentation techniques using measures such as BDE(boundary displacement error),...

11 October 2018 2,021 5 View

Most appropriate Feature Selection to find indicators that are most correlated to the number of one event?

I have two databases. One of them contains the number of this event per year. The other one contains a lot of indicators and its values per year. I want to find those indicators that are most...

20 March 2018 3,610 4 View

Which is the best way to treat ticks and fleas for morphological identification before performing molecular investigations?

Hello all, I am trying to attempt morphological identification of ticks and fleas collected in Wales on wild small rodents. My samples have been frozen and after the morphological identification I...

10 October 2017 7,374 4 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Do I need to ask permission to use pictures for my questionnaire?

I would like to use some photo from a government website for my questionnaire. But, different photo is from the different owner. So, should I just get permission from that government body or still...

23 February 2021 1,631 3 View

No title

The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

Onset of Photo Oxidation in Polymers?

Hi! I am looking for some specific information in regards to the minimum amount of UV intensity required to begin the photo degradation process in polymers. I understand each polymer is...

15 February 2021 7,703 4 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

What form of data analysis should I use for my pilot study?

I am currently conducting my final year dissertation which is looking at the efficacy of common police lineup practices. However, before I launch my study, I have put out a pilot study which is...

08 January 2021 4,376 4 View

Any suggestion on the methodology for Soil depth and ph mapping using machine learning ?

Hello, Please, I need your opinion. I want to map the depth of 20,000 hectares of land. I have sampling points of 20,000 with their respective depth attributes and photos of the topsoil(less than...

31 December 2020 4,325 3 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

ESA Eureca spacecraft Solar Array Arcing demage, What is the cause of the problem?

We see a photos of solar array damage suffered by the ESA Eureca spacecraft that was recovered by the Space Shuttle. photo shows a sustained arc site on its solar arrays. In this case, the...

23 December 2020 2,512 2 View

Why bands with lower sizes appear in higher MWCO filter?

I performed Tricine SDS PAGE according to the Nature protocol. Included in the gel is 4% stacking, 10% spacer, and 16% resolving. I intended to separate peptides lower than 10kDa. Therefore, I...

16 December 2020 1,578 1 View