Hello,
I am working on production and purification of functional amyloids, that are also involved in biofilm formation. The thing is that to identify amyloid producing strains I would like to have a quick preliminary method. My lab has worked with Salmonella and E. coli and they use T agar supplemented with Congo Red and red colonies indicate amyloid production (in this case curli). I will be working with other species, such as, Pseudomonas, Streptococcus and Staphylococcus and I am not sure if this method will work for these bacteria. I saw that it has been done for Pseudomonas, but I got confused with Staphylococcus and Streptococcus since for Staph I have seen that if you do Congo Red for biofilm formation due to exopolysaccharides, colonies will be black so I wonder if I should look only for red colonies or if this does not apply at all
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