I am currently researching modularity in the PFC. I have used adult male rodent brains which were stored in sucrose prior to use. I understand that all animal procedures were in strict accordance with the animal care guidelines. However, would euthanising the rodent and then using the brain directly change the experimental findings?
Thanks
You raise a key question in experimental studies. You principal scientist.
You learn stress. You understand that in any case, in conducting all the rules of euthanasia, all animals (in the experimental group and the control group) will experience at least in some measure marked stress. Stressor - a procedure anesthesia, hypoxia, sectional procedure. Living organisms will react to all these manipulations. Since all of these factors will be the same in the experimental and control groups, the differences will be identified due to a stressor test that you used in the experimental group. So, you need honesty and meticulousness in the experiments, the strict observance of all experimental conditions. Then you will be able to identify differences in the experimental and control groups.
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