What is the best method for prebiotic delivery to mice? dose of prebiotics? and treatment period?
https://www.jove.com/pdf/59074/jove-protocol-59074-probiotic-studies-in-neonatal-mice-using-gavage
Humam Kasem Hussein thank you for your answer. Do you think prebiotic could be delivered by gavage as well. I am working with prebiotic not probiotics.
Yes you can, so you will study the effect of such as inulin or other prebiotics on microbiota gut microorganism in animal or human modules
Nihan Beyatli Thank you. I am planing to study the effect of the prebiotic using mouse model. Any suggestion about dose and treatment period?
You can intake diet 3-4 g/mouce day for 3-4 weeks then study factors, body weight ....ect
With my all best wishes
Nihan Beyatli thank you
I would like to know what is the best dose of levan to use in vivo and the treatment period. I am using mice and I would like to gavage them with levans.
24 March 2021 8,113 4 View
I was wondering if an increase in neurotransmitters such as NE will lead to an increase of B3AR expression?
08 July 2017 5,020 1 View
I was wondering if therer is any drug that disrupt autophgy in vivo. I would like to inject some transgenic mice with this drug and see how autophagy is affected. Thanks
29 March 2016 1,487 3 View
I would like to know how to activate cathepsin in cells.
31 January 2016 3,764 3 View
I am using Tunicamycin as ER stress inducer in vivo and it is reducing the body weight a lot.
21 January 2015 7,592 0 View
Is there any reliable method other than mass spectrometry ? Cholic Acid
08 December 2014 5,328 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View