Cell infection with lentivirus: GFP+ after soring by flow but no protein expression?

I use a plasmid to overexpress 1 protein in 293T and murine BMSC. But I could never succeed. I produced the lentivirus by transfecting the plasmids in HEK293FT cells, together with another 3for...

24 December 2020 6,389 3 View

I keep getting an error for GEOPIV on Matlab?

When doing the geoPIV_RG_run, i keep encountering this error. Error using dataread Trouble reading floating point number from file (row 1, field 2) ==> Dynamic Load Test Free Vibration Error...

20 October 2020 10,004 2 View

Is lentiviral production of antibodies in 293T viable?

Hi, I recently started using lentiviral systems to produce proteins in mammalian cells. Knowing the performance of this impressive system in producing high titers of proteins, I am wondering if...

05 August 2020 5,144 2 View

What solutions do you suggest for molecular field visualization/analysis?

Hello, I wonder what options exist these days for visualizing "molecular field" type data. That is, a 3D scalar field represented by translucent volume rendering or isosurfaces, and overlaid on a...

12 June 2020 861 2 View

How to choose collective variables for ligand binding to protein?

Hello! What is the recommended way to choose collective variables / reaction coordinates to study ligand binding to a protein (e.g., for PMF free energy profiling)? I often see the advice like "Do...

10 March 2020 1,878 4 View

No title

02 November 2019 8,943 3 View

Why an SD of means difference between two groups (e.g. treatment vs. placebo) is equal to a particular SD in each group?

Very frequently in papers, devoted to parallel clinical trials, I face a situation, when a calculated SD for an effect in each group is approximately equal to an SD of effect difference between...

07 July 2019 9,217 4 View

What aproach we must use to calculate force in electrostatic problems: potential or electric field intensity?

Well known problem of finding potential for metal sphere and point charge, solving with "mirror image" trick give you 2 different answers for the force, acting on point charge. One when we use...

14 December 2016 9,365 5 View

Does choline hydroxide (46% aqueous) ionic liquid still absorb water from surroundings?

I will be using this ionic liquid as an electrolyte for my battery experiments.

01 August 2016 2,559 1 View

Hall Sensor and EMC?

What would be your brief recommendations for EMC for Hall sensors? I mean; how to increase robustness of the Hall sensors circuits? Any links? Thank you, Eugene

27 April 2016 8,388 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View