Do you need to acidify your samples prior to desalting with C18?

I am wondering how important pH is here. I have a sample tryptic sample in 50mM TEAB, wondering if I need to add in TFA to ~2% before desalting. I understand the need to acidify samples with ion...

18 October 2020 6,639 3 View

I'm looking for ways of analysing discoure complition tasks and ways of devising them, any suggestions?

I am an MA student working on pragmatics, specifically DCTs (discourse completion tasks). do you happen to know any books or articles on how to write and analyze DCTs? or is there any source for...

28 July 2020 5,136 2 View

Do you know any solvents to remove insects from the sticky traps?

We have been using thinner to remove the insect specimens from sticky traps. However, we would like to use other solvents with no toxic effects.

15 March 2020 6,789 12 View

Does anybody have an Android ".csv" Malware Data-set?

17 December 2019 761 3 View

Probelm with expressing hetrologous proteins using Pichia pastoris?

07 December 2019 7,502 6 View

How to calculate flat membrane pore size distribution using membrane DI flux?

Almost all membrane pore characterizing techniques cannot be used due to the mechanical instability of the flat membrane. I want to use deionized water flux (DI) and mathematical way to determine...

04 December 2019 3,850 2 View

Which part of polypeptide is susceptible for reaction?

A given polypeptide protein has side chains and terminal (N- and C-). For any modification or conjugation reaction which part involved in reactions (side chains or terminals). Thank you !!

17 October 2019 575 2 View

For many days enzyme expriment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced ?

For many days enzyme experiment, fresh medium of enzyme need to be replaced regularly. At what time interval the medium should be replaced by new to keep activity of enzymes?

01 September 2019 7,619 4 View

I run GSSHA model in WMS software but the result was not reasonable what is the problem?

i do it with 32 mm precipitation

05 August 2019 8,187 2 View

Hi, i want to work in targeted cpp-mediated delivery of pDNA. but i do not know if DNA can stably reach to nucleus?

i want to design a cell penetration peptide for delivery of pDNA. that this platform targeted with a tumor targeting peptid

24 April 2019 6,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View