I am wondering why you don't (normally) run post-hoc analysis after insignificant interaction effect from a Two-Way ANOVA even if there is a simple main effect?
If there is a significant interaction effect, then the post-hoc on the main effects are often not of interest. But the post-hoc on the interaction is of interest.
If there is not a significant interaction effect, but there are significant main effects, then the post-hoc is performed on the significant main effects.
I have been told by previous researchers who tell me that there is no point in running a post-hoc analysis if there is no significant interaction effect between two predictor variables
I never run or interested in running post-doc analysis for insignificant interactions as well. Even you run this, then effect will be seen in the same direction and only your significant main effect will have a role there, I think.
If there is a significant interaction effect, then the post-hoc on the main effects are often not of interest. But the post-hoc on the interaction is of interest.
If there is not a significant interaction effect, but there are significant main effects, then the post-hoc is performed on the significant main effects.
Thank you Salvatore and Prabhat. You have both clarified it well for me. I believe I understand what you both are saying. So even if there is no significant interaction effect but there are significant main effects, then I would only do post hocs for those groups within each main effect, correct?
Wei et al (“Comparisons of treatment means when factors do not interact in two-factorial studies”, Amino Acids, 2012 42(5):2031-5) provide examples of 2-factor studies where it is useful to perform a post-hoc analysis when only one factor and not both factors or their interaction is significant. In the abstract, they wrote "when the two factors do not interact, a common understanding among biologists is that comparisons among treatment means cannot or should not be made. Here, we bring this misconception into the attention of researchers. "
Instead of focusing on p-values, take into consideration effect size comparisons. You'll find theoretical and practical implications in:
Becker, B.J., 1988. Synthesizing standardized mean-change measures. British Journal of Mathematical and Statistical Psychology 41(2), 257-278.
Jasbi, M., Sadeghi Bahmani, D., Karami, G., Omidbeygi, M., Peyravi, M., Panahi, A., Mirzaee, J., Holsboer-Trachsler, E., Brand, S., 2018. Influence of adjuvant mindfulness-based cognitive therapy (MBCT) on symptoms of post-traumatic stress disorder (PTSD) in veterans - results from a randomized control study. Cogn Behav Ther, 1-16.