23 June 2017 1 1K Report

Looking to reduce the metabolic rate of HepG2 cells.

More Kiera Ward's questions See All
How i can intersect 2 Achses in Amira or 3D Slicer ?

English: Hello, I'm working on a doctoral thesis in the field of the cochlea. I've reached a point where I need to define axes on a segmented cochlea. To do this, two template axes in 3D space...

12 December 2023 6,223 0 View

Segmentation with Amira-Avizo?

Hello, I would like to ask if anyone can help me : I have to segment a cochlea first and later measure the volume using Amira software as part of a PhD thesis. Could someone tell me how to do...

24 September 2023 274 2 View

Imaging immunofluorescence stained histology on Zeiss AXIO with HXP 120 V laser. Unexplainable striping on image due to laser?

Hi all, I'm trying to image fluorescent stained spleen and I seem to be getting some aberrations on the images, a kind of striping effect. I suspect it is from the laser. Channels affected are...

31 January 2023 5,897 3 View

Protease Q: Process human synovial fluid samples for protease analysis and it turns to jelly. Think DNA is the cause, any suggesitons on how to fix?

Processing synovial fluid samples for protease analysis with a mass spectrometer. Samples are centrifuged at 4 degrees 2200g for 15 min, then heat-denatured at 95 degrees for 10 minutes. Samples...

23 January 2023 2,945 3 View

Bacteiral protease activity using fluorescence tagged peptides?

I am trying to calculate the amount of peptide I would need to order to complete a bacterial protease activity assay using fluorescence. I hoping to look at E. coli and S. aureus strains with...

09 January 2023 9,934 2 View

Have I set this up correctly to determine sample size using G*Power?

I am working on my first educational design research study and trying to determine what the minimum sample size should be. This is a pretest posttest model where the same students take a...

16 December 2022 9,548 1 View

How can I determine genetic interaction based on Arabidopsis root growth rate measurements?

I have a series of root growth rate measurements of Arabidopsis lines carrying a single KO in gene x or gene y, and a series of measurements of lines carrying a double KO in both gene x and y. I...

03 November 2022 5,868 0 View

How do I perform statistical tests on multiple response sets in SPSS?

I have conducted an online survey and I want to run a statistical test to determine of there is any correlation between, for example: 1. The number of years of experience a person has (selected...

05 June 2022 9,284 2 View

Is thawing and re-freezing protein samples a necessary evil or is it best to aliquot 3> times?

Hello, I have 100 tissue samples (50 cortex and 50 hippocampus) that have been homogenized (sigma RIPA + sigma protease inhibitor (PI)). I have made three supernatant aliquots per sample. I...

05 June 2022 8,092 0 View

How do you download ADCP data in csv or ASCII format using Pulse?

We only seem to be able to download .ENS files in section "Download Data" in Pulse, but we would like to have csv or ASCII.

19 October 2021 1,364 1 View

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Why activated CAR-Jurkat cell could not kill targets?

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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

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How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

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Best mask design for metal deposition aiming build a Perovskite solar cell device?

What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?

31 July 2024 4,835 3 View

How to culture and maintain Lx-2 cells?

Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...

31 July 2024 3,893 0 View

How to treat monolayer culture cell line with essential oil?

Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...

31 July 2024 5,301 2 View

Creating an Automaton/Using Language as the Model?

As animals learn a task, they become more reliant on their long-term memories as compared to the real-time sensory information to guide behavioral performance (Ahilan et al. 2018). This process...

31 July 2024 9,859 0 View

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