How do I change a preprint to published?

I want to know how to change the status of an article.

21 February 2021 1,536 2 View

How do you calculate a discrimination index for polytomous data?

Hello, I'm currently running a PCA on a polytomous data set (5 point Likert). I want to create a discrimination index to narrow down my items before running the PCA. After looking around, I'm...

13 February 2021 3,517 2 View

Has anybody made their own NEB Stable competent cells?

I'm a cheap-skate and don't want to keep buying the NEB stable shots. I regularly make my own XL-10, DH5 and BL25 (Hanahan protocol) competent cells, but am wondering if anybody has any experience...

08 July 2020 1,262 5 View

Glucose assay in HepG2 cells ??

hallo every one, i need a protocol to measure glucose production in HepG2 cells, actually I need a detailed Protocol that I can based on as a starting point for my research, thank you in advance,

10 March 2020 4,620 4 View

Do you work in primary education in the UK?

If you work in primary education or with primary aged pupils either who have or do not have SEN. Then would you consider filling out my questionnaire as part of my dissertation into professionals...

23 January 2020 5,434 3 View

Professionals' interpretations and definitions of inclusion a good dissertation idea?

I have recently just started the 3rd year of my degree and I am struggling with my topic. I have an idea which I have been told would be interesting and make a good topic but I am struggling with...

15 November 2019 2,775 5 View

What to do if Levene's test is significant in a mixed ANOVA in SPSS?

Hi everyone, As part of my PhD project, I am conducting mixed ANOVA's in SPSS. I understand how to run the procedure and have been using the SPSS Survival Manual by Julie Pallant as my main...

23 November 2018 5,629 5 View

A paired sample t-test for unequal sample sizes?

I understand that if you collect data pre and post-intervention from the same individuals, a paired sample t-test is the best option to compare differences between the scores. However, if a few...

29 July 2018 5,356 4 View

What are the correct values to use for stepwise backward regression from an intervention study?

I have conducted a human intervention study and measured various physical and metabolic characteristics pre- and post-intervention. I would like to conduct stepwise backward regression in SPSS to...

17 January 2018 2,785 8 View

Conferences relating to physical inactivity, sedentary behaviour and health taking place in 2018?

Looking for recommendations of highly regarded academic/research conferences on physical inactivity, sedentary behaviour and health taking place next year in 2018.

05 November 2017 1,549 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View