Possible uses of refractometer?

11 November 2015 1 3K Report

Refractometer can be used to find dn/dc values for SLS. Is there any other inference that could be drawn from measurement of refractive index? Possibly by changing the wavelength or temperature?

V. N. Ravi Kishore V.

One could use it to determine the exact concentration of urea/guanidine hydrochloride/sucrose solutions which have been prepared long back and stored.

This is especially important in experiments where exact concentration needs to be known accurately (used in biology).

Badges
Science topic

More Mithra K's questions See All

FCS focal volume calibration ?

I am trying to calibrate the focal volume of FCS setup by using Alexa 488 dye. The experiments is being carried out at room temperature (25 degrees). I have to take the diffusion coefficient of...

05 June 2019 5,600 1 View

Optimum dilution of microgels for FE-SEM imaging?

What would be the optimum dilution of microgels that should be used in the case of FE-SEM sample preparation? I would be using a glass substrate for imaging. I will either spin coat or dry the...

03 April 2016 10,017 4 View

Anyone familiar with the Hofmeister series, arrangement of ions?

In the typical Hofmeister series for ex F->Cl->Br->I-(Ionic radius 0.133,0.181,0.196,0.220 respectively), The ions on the left of Cl- is a Kosmotrope, whereas the ions on the right are...

11 December 2015 7,706 2 View

Turbidity- What wavelength technique determines the N value and hence colloidal stability of microgels?

Turbidity-wavelength technique is used by many to determine the 'n' value and hence the stability of the microgels. The formula used for the same is n=-(dlog tur/dlog lambda). Here turbidity can...

10 November 2015 1,670 5 View

Any advice on DLS experiments of microgels?

I have prepared a microgel (solvent water) and during the process I have added NaOH to maintain the pH of the solution as 12. Microgels have formed. Now when I do DLS experiments I saw that the...

09 October 2015 8,404 6 View

Mole percent to grams?

How do I convert mole percent to grams? Is this calculation correct? If I have 15 mol% A and 85 mol% B. Then mass of A needed= (15*Molecular weight of A in g/mole)/100 ? (x g) and mass of B...

07 August 2015 8,157 0 View

NaOH solution preparation?

I know it's kind of a basic question but how do I prepare a solution of NaOH(pH=12)(100ml)? I have done the calculation but seem to be not getting the result.

07 August 2015 372 1 View

Polymer hydrogel preparation ?

Is anyone working or is aware of a hydrogel which can be prepared at room temperature? (Without heating it to higher temperature above room temperature Ex: PNIPAM hydrogel )

06 July 2015 9,602 3 View

Why do I get microgel particle aggregation?

I have prepared microgel particles whose size should be around 100-150 nm. I have filtered the sample with the help of 250nm filter. But when I am carrying out the dynamic light scattering...

06 July 2015 4,708 9 View

Anyone familiar with PNIPAM microgel rheology?

In the figure given below, they have measured the relative viscosity of the microgels by varying the polymer concentration c(wt%). I had a doubt that, here whether they have prepared different...

04 May 2015 6,564 9 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

How to increase citation in Research Gate?

How to enhanced h-index in Research Gate?

04 August 2024 3,368 4 View

Is it possible to improve the reforming section of the Haber-Bosch process without using air separation units?

A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...

01 August 2024 7,051 5 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

H-index issues?

Hi Sir/Madam, I would like to know why my h-index is stuck to 15 despite the number of new publications added. Could you explain me how marks are granted. Best, Daniel

28 July 2024 2,607 2 View