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Questions related from Mithra K
I am trying to calibrate the focal volume of FCS setup by using Alexa 488 dye. The experiments is being carried out at room temperature (25 degrees). I have to take the diffusion coefficient of...
06 June 2019 5,558 1 View
What would be the optimum dilution of microgels that should be used in the case of FE-SEM sample preparation? I would be using a glass substrate for imaging. I will either spin coat or dry the...
04 April 2016 9,977 4 View
In the typical Hofmeister series for ex F->Cl->Br->I-(Ionic radius 0.133,0.181,0.196,0.220 respectively), The ions on the left of Cl- is a Kosmotrope, whereas the ions on the right are...
12 December 2015 7,669 2 View
Refractometer can be used to find dn/dc values for SLS. Is there any other inference that could be drawn from measurement of refractive index? Possibly by changing the wavelength or temperature?
11 November 2015 3,080 1 View
Turbidity-wavelength technique is used by many to determine the 'n' value and hence the stability of the microgels. The formula used for the same is n=-(dlog tur/dlog lambda). Here turbidity can...
11 November 2015 1,634 5 View
I have prepared a microgel (solvent water) and during the process I have added NaOH to maintain the pH of the solution as 12. Microgels have formed. Now when I do DLS experiments I saw that the...
10 October 2015 8,282 6 View
How do I convert mole percent to grams? Is this calculation correct? If I have 15 mol% A and 85 mol% B. Then mass of A needed= (15*Molecular weight of A in g/mole)/100 ? (x g) and mass of B...
08 August 2015 8,119 0 View
I know it's kind of a basic question but how do I prepare a solution of NaOH(pH=12)(100ml)? I have done the calculation but seem to be not getting the result.
08 August 2015 333 1 View
Is anyone working or is aware of a hydrogel which can be prepared at room temperature? (Without heating it to higher temperature above room temperature Ex: PNIPAM hydrogel )
07 July 2015 9,563 3 View
I have prepared microgel particles whose size should be around 100-150 nm. I have filtered the sample with the help of 250nm filter. But when I am carrying out the dynamic light scattering...
07 July 2015 4,659 9 View
In the figure given below, they have measured the relative viscosity of the microgels by varying the polymer concentration c(wt%). I had a doubt that, here whether they have prepared different...
05 May 2015 6,525 9 View
I have synthesized PNIPAM microgels, and it undergoes a transition at around 32 degrees. When I do DLS of these microgel particles, the intensity(counts) is more for dehydrated state(after 32...
03 March 2015 4,304 10 View
In the formula for the turbidity measurement from UV-Vis data, it is given that turbidity=(2.3*A)/L, where A is the absorbance and L is the optical path length. Can anyone tell me why there is a...
03 March 2015 3,094 6 View
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01 January 2015 4,563 4 View
Does anyone know the procedure of removing the inhibitor from the monomer acrylic acid? Is it necessary to do so in order to achieve polymerization?
09 September 2014 6,372 10 View
Thanks in advance.
08 August 2014 7,770 9 View
I wanted to quote a few examples of chemical hydrogels which posses a neutral charge.(non-ionic). Some examples I have are PEG,PEO,PVME etc. Are there any other polymer hydrogels which posses...
07 July 2014 9,895 6 View
In FCS, the expression of diffusion coefficient is D=W2/4T(tau). But when I referred a paper for simplicity they had calculated the diffusion coefficient for 2D and had derived the above...
07 July 2014 7,500 6 View
For example: If you consider agarose, by seeing the structure is it possible to predict whether it is charged or neutral when added in water? I have attached the image of structure of agarose....
07 July 2014 2,592 7 View
While reading a paper I came across a method to prepare a hydrogel. It mentioned that the fully hydrated suspension was incubated in a sealed flask at 95 °C for 8 h. Is it equivalent to heating in...
05 May 2014 3,788 7 View
01 January 2014 4,225 5 View
