Possible experiment to check protein solubility in cell culture model?

Dilshan Harischandra @Dilshan_Harischandra

09 September 2018 1 9K Report

I am looking to analyze how the solubility of aggregation-prone proteins (like Tau, A-beta or a-synuclein) behave under different protein disaggregases and chaperons. Something along the line of how solubility of a-synuclein or Tau changes under forced expression (transient transfection) of protein disaggregase (Hsp104) in cell culture system. Importantly how chaperons or protein disaggregase change overall protein solubility in cells.

.... any thoughts?

Steingrimur Stefansson

Hi Dilshan, one problem that I foresee is that many intracellular misfolded proteins are ubiquinated and degraded by proteasomes.

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