24 March 2023 0 326 Report

Hi Fellows, I am trying to knock out a essiential gene. I have knock-in a stable mutant copy into cell by lentiviral infection, I would to subsequencially remove the endogenous copy by CRISPR-cas mediated KO, is there any way to design the sgRNA? should I make it target at UTR or exon-intron junctions? Any fellow have ideas?

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