27 January 2024 1 10K Report

Hi,

I had stained CD40 antibody on tonsil paraffin sections. But staining came positive only on periphery of tissue and there is no staining in the cente of tissue. What can be the reason??

More Aarti Kanzaria's questions See All
how to fit Hikami-Larkin-Nagaoka formula in origin?

I have resistance data on y axis and magnetic field on x-axis? how to fit HLN equation on this data using origin?

19 December 2023 6,704 0 View

Why does my direct effect between my IV and main DV is insignificant?

Dear community, I am working on my thesis data and my data analysis shows that there is no relationship between IV and DV, only the indirect relationship exist in presence of 2 mediators (serial...

16 September 2023 1,505 4 View

Could someone please suggest me any method to get the list of buried water and surface water in md simulation output files?

my system having proteins , organic molecule and water

12 September 2023 5,489 1 View

rfid tag as a temperature sensor?

Anyone having experience in designing rfid tag as a temperature sensor?

04 May 2023 7,187 3 View

Hello Reseachers , can you please suggest me some methods or anything to analyse the clustering of organic molecules in MD simulation?

I did the MD simulations of my interest of protein with some organic molcules like Riboflavain and they making cluster near the protein and i want to find different cluster size vs probability...

01 May 2023 8,494 2 View

Please suggest me a suitable test, if an independent variable is measure on 5 point Likert scale and dependent variables is categorical variable?

Respected all My dependent variable is saving behaviour measured as a categorical variable. My independent variables are 11 saving motive statements measured on 5 points Likert scale (5=strongle...

22 November 2022 5,635 5 View

1. In descriptive survey research Can I use t test without developing a Hypothesis? 2. Can hypothesis be developed for one objective only?

Kindly Provide your suggestions its an important part of my research work.

30 May 2022 3,191 6 View

Use of chi-square test?

Respected all, I want to clarify, 1) can I use the chi-square test to check the association between gender and Retirement confidence ( measured as Yes, No, Not calculated) and Gender and...

04 February 2022 9,347 5 View

How to predict evapotranspiration (ET) using land use land cover changes (LULC)?

I have LULC and ET data for the past three decades and have predicated LULC for future time period (e.g., 2025-2055). Now, I want to predict ET using land use land cover changes.

07 December 2021 4,872 11 View

Any one can help me for YAFS simulator?

Please help me. Is there any video for how work on YAFS

25 September 2021 7,488 3 View

Similar questions and discussions
What is the reason for current dropping in OER , LSV curve?

I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...

10 August 2024 3,629 1 View

What may be the reasons for failures of Tube toi Tube Sheet Joints in Boiler Drum ?

We have observed that tube to tube sheet joint leaked in our boiler and needs to overcome same by knowing the root cause.

08 August 2024 3,161 0 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to fix errors in my heat transfer steel structure with reinforced concrete slab model Abaqus?

I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...

07 August 2024 1,028 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

What should I do with parameters that are not relate to my simulation in MyLake model?

I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?

04 August 2024 1,537 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close