Lipid structures disappear upon negative stainging. Does anyone knows this effect?

13 December 2020 1,559 3 View

Cryo-EM Vitrobot settings for large lipid structures. How can I reduce ice thicknes?

Dear all, I have an issue figuring out good plunging conditions for large lipid structures: I have carbon coated C-Flat 2/2 400mesh copper grids with large (elongated) lipid structures on it....

13 December 2020 6,696 2 View

Can I use lentiviral packaging HEK293T cell lines to produce stable clones?

Hi, I generally made LV in HEK293T cell lines by triple transfection. I am wondering whether the newly generated LV will transduce the packaging HEK293T. In this way I could exploit the...

09 September 2020 6,333 3 View

Which software is the best for scientific presentations in which equations occupy a big part of it?

I usually use Beamer, in which equations are easy to write (and I already have them in Latex) but it's too static and rigid. In Powerpoint it is impossible to write the equations, it takes a lot...

15 July 2020 8,260 3 View

How to calculate the max Jsc for a given Eg using spectral irradiance [W/(m2*eV)] data?

I'm calculating the max available Jsc as a function of bandag Eg. For that I'm using the following integral: Jsc=q*int(f(Eph)*EQE.dEph) from Eg to \inf With: q=1C/eV f(Eph) Spectral Irradiance...

07 July 2020 5,873 4 View

Inverted current Mirror connection in LM741?

Analyzing the topology of the LM741 , the current mirror (Q8-Q9) that bias the first stage of the op. amp. is not connected as usual (see the red circle on the picture). Curiosly, this way to...

24 January 2020 8,419 2 View

Negative values in anti-inflammatory assay: inhibition of albumin denaturation?

I am performing in vitro anti-inflammatory assay: inhibiton of albumin denaturation by organic extract and purified fractions from natural material. Since my samples are colored (red, orange,...

14 September 2019 2,528 1 View

Can you help me with Python script?

Hi to all, I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file...

05 February 2019 2,494 1 View

What are good color palettes for students in the autism spectrum?

I've read that, for students in the spectrum, it may help to design a class environment that avoids overstimulating their cognitive system with unnecessary distractions, one of them begin colorful...

28 January 2019 2,557 3 View

How to improve undergraduate and postgraduate educational settings with gamification?

Gamification is a brilliant strategy to support the development of educational settings, especially in undergraduate and postgraduate courses. Therefore, I would like to ask you what do you think...

22 October 2018 2,456 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View

Specify the Burgers vectors for dislocations, which cannot be uniquely identified.?

1. A bcc metal single crystal was sliced along (1 -1 0) plane for TEM study. Assuming that the TEM available is equipped with a goniometer stage which tilts up to 45o. The Burgers vector of...

20 February 2021 2,370 1 View

I am working on image transmission, i converted the image into binary vector, i want to extract the MSB of each pixel ?

i have a vector 1*512 size, i want to take the first value every 8 elements and put these elements at the end of the vector,how to do this on matlab ?

19 February 2021 2,352 5 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View