PolyA signal on lentiviral vectors?

17 November 2021 1 7K Report

Hi, I am making a 3rd generation LV expressing my gene of interest. I know that putting a polyA signal within the LV genome will lower the vector titer, but what happens if I put it in the opposite direction? Did anyone try?

Di Xia

Yes, use antisense (opposite direction) if you do need to put a polyA in the middle. Take the following research as an example.

https://www.nature.com/articles/gt200946

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