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Hi to all, I have an approaching deadline and to perform my analysis (design guide RNAs) I need to insert a lot of mutations in my sequenze (txt file). Mutations are inside a excel in file...
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I engineered an eukaryotic promoter inserting the LacO sequence. The system I designed renders the expression LacI dependent……my problem is that the expression is effectively silenced by LacI, but...
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In my petri in which I can identify my colonies harbouring the plasmid I propagated, I saw some colonies red and yellow (sometime even black) growing in the same petri. They grew with delay...
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