I would like to know ,
a. regarding the concentration of PLL (tried 0.1 & .01% - cells didnt attach)
b. whether Poly D lysine is better than Poly L lysine in terms of cell survival.(0.1% - most of the cells were dead)
Any suggestions...
Are you coating plates? Generally it depends on the cell line whether you use Poly-L-Lysine or Poly-D-Lysine. For example, PC12 cells will attach better to plastic if you coat it with Poly-D-Lysine. For these cells I incubated plates beforehand with the poly-D-lysine soloution (I think 0.05 mg/ml although this was a long time ago so I may have mis-remembered that) and then washed at least once with PBS before addition of cells. Alternatively you can dry the plates/flasks and leave at 4C for a while.
Dear
Cell lines/cell types release proteases can break poly-L-lysine, in this case it is more convenient use poly-D-lysine. So poly-D-lysine.is better to be used.
You can check the following link: http://www.coleparmer.com/TechLibraryArticle/571
Cordialy
Marius
To help facilitate attachment, cell spreading, growth, morphology, differentiation PDL or PLL are used. In histochemical applications both polymers of D- and L-lysine are used to coat slides to promote attachment of cells. Poly-L-lysine has also been reported to improve protein coating of ELISA plates. Poly-Lysine enhances electrostatic interaction between negatively-charged ions of the cell membrane and positively-charged surface ions of attachment factors on the culture surface. When adsorbed to the culture surface, it increases the number of positively-charged sites available for cell binding. for the concentration of PDL (Its really good) 10mg/ml
We tend to use PLL but it's a bit finicky for larger plates. However, we use fixed dead cells so this might not help you
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