Does anyone know how to remove a hardened agarose gel from an imager?

Our lab discovered a gel that was left overnight on our alphaimager with the UV light left on. It is now rock solid and very thin, fused to the surface of the imager.

01 February 2021 6,774 2 View

Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to slides?

Is any way or method to seal agarose pad or channels fabricated agarose gel pad to glass slides as like we do plasma bonding with PDMS to glass slides?.

04 January 2021 3,589 2 View

Comparative STUDY: sample of sector A in 5 cities and sector B in whole country for ?

sector A sample is 313, and sector B sample is 200. If I take sector B population in the whole country then sample B's comes = 330 Q. Can I take sector A in 5 cities and sector B in the whole...

27 December 2020 6,930 2 View

Find value of heat transfer coefficient in SI units??

A tube is subjected to air at standard temperature and pressure conditions with velocity 5 m/s. initial temperature of the tube is 980 K and drops to 500 K after interaction with the cool air....

21 December 2020 6,408 3 View

Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?

Can we transform any gfp tagged plasmid vector in to any E.coli strains with a objective to visualize E.coli bacteria as fluorescence molecule?. I need a valuable suggestions so Please help me....

30 November 2020 2,469 8 View

No title

23 November 2020 6,277 5 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

How can we define/simulate shell elements (S3/S3R, S4, S4R, SAX1, SAX2) in ABAQUS using a python script while defining bending behavior of composites?

Working with ABAQUS 6.14 for defining the UMAT subroutine for the initial imperfection of shell elements.

19 October 2020 974 1 View

How to dry nanoparticles without aggregating?

After freeze-drying my synthesized nanoparticles aggregate and do not disperse completely in water. Please help

05 October 2020 2,233 6 View

How to disperse freeze dried nanoparticles?

After freeze-drying, the nanoparticles could no longer be dispersed completely in water. Any suggestions on how to do it?

05 October 2020 7,814 4 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View