Please suggest troubleshoot for northern blot by fluorescent probe (cye5). How did i get small RNA band complementary to my probe in Nylon membrane ??

01 January 1970 0 4K Report

I am trying to do northern blotting of small RNA (E.coli) using RNA blot(Bio red).Total RNA had loaded in 6% urea gel and transferred to a membrane (Amershon- H+ Nylon membrane-GE health care). There was no band in gel after transfer. After pre-hybridization then hybridization with complementary probe (cye5 labbeled) and scanned under phosphorimager. The membrane showed complete copy of total RNA band as shown before transfer.

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