Nivas Manohar Desai
1. Apply the diluted suspension to a coverslip.
2. apply the diluted suspension to a layer of fir balsam previously prepared on a cover slip
3. prepare a layer of suspension with a dried solution of polymer material
Similar to what Yuri have suggested. Depending on your nanoparticles, it is probably best to centrifuge your sample to get more of the NPs when put it on a clean substrate. You can use silicon wafer, coverslip, or any clean substrate to hold your NPs. Here's what I would try:
1. suspend the NPs in ethanol. then centrifuge.
2. discard most of the liquid after centrifuge.
3. take 5 uL of the NPs and put it on Si wafer.
4. left to dry and should be ready for AFM.
You could do a quick way to prepare your NPs thin films by drop-casting on a cleaned slides. Alternatively use a small doctor blade to get better films for AFM or high resolution microscope which does the job too.
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