Please suggest kit for isolation and purification of NK cells from sources like peripheral blood, bone marrow.
Also suitable expansion media for NK expansion without losing the phenotype CD56+ CD16+ CD3-
References will be highly appreciated
Hello,
here is a negative (untouched) isolation method for NK cells.
https://www.pluriselect.com/product-details/product/plurispin-human-nk-cell-enrichment.html
maybe it helps you.
BW
Michael
Miltenyi Biotec Nk cell separation kit for negative selection, reasonable purity output
http://www.miltenyibiotec.com/en/products-and-services/macs-cell-separation/cell-separation-reagents/nk-cells/nk-cell-isolation-kit-human.aspx
for activation
http://www.miltenyibiotec.com/en/products-and-services/macs-cell-culture-and-stimulation/stimulation-reagents/stimulation-based-on-macsibead-particles/nk-cell-activation-expansion-kit-human.aspx
for expansion IL-2 can give you similar effect at 50-100 IU/mL in RPMI 5% FCS plus 5% AB Sigma human serum.
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