I've been measured the percent of my concentration series (R2 = 0.998) but the data weren't covered value more than 50%.
Should I repeat my experiment or I just extrapolate the regression formula to 50%.
Thank you very much for your answers
You do not have to repeat all of the experiment
Just complete the experiment by testing higher concentrations
best regards
Hello, the same topic is well discussed here.
https://www.google.com/amp/s/www.researchgate.net/post/Can_someone_please_tell_me_ic50_value_of_following_readings_of_my_dpph_assay/amp
Good luck!
Thank you
Thank you Dr Nasher
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