would you please inform me if I can detect proteins from serum frozen at -80 C and never thawed
hi
I do not think it's a problem, except for proteins :
1- that have a low stability
2- short half life
3- if you want to work on proteins function
For example, you will not have a problem with Albumin, but you will probably be difficulty with coagulation factors ( such as FVIII or FVII ).
It's completely different from protein to protein.
good luck
what about myokines
what about irisin ?
I have no experience in this. But it's probably a problem with myokines, which is a small cytokine, but I do not think it's an issue with the Irishman. Compare the test for more certainty with a fresh sample and a frozen sample.
you can easily use pre-test before main analysis.
just use the small part of your stocked solution for protein assay with new blood sample from the same species with same conditions .
Thanks a lot for the answers
You should have no problem for an sort of protein and DNA assays .
Dear all Good evening I am asking if you have experience with publishing scientific papers in Change management, organizational development, project management, or business management I need...
19 July 2024 9,461 4 View
I need help with one survey to reach 3000 people (response). I will be really thankful if anyone can reply and share this...
01 February 2024 1,437 7 View
I have made a model in Abaqus program. I want to define "E" as a constant value at each node in the part. I have entered in inp. File *Depvar 1, *Elastic, dependencies=1 1000., 0.25, ,...
29 April 2023 1,351 8 View
the model has three diversity types as independent variables and a team process in the middle (1 variable). however there is no hypothesis of being a mediator and then 1 dependent variable...
29 April 2023 1,419 0 View
I have a code to get data from ABAQUS by using MATLAB. How do I convert this Python code from extracting data at integration points to unique nodal? "from odbAccess import * from sys import...
07 April 2023 3,756 0 View
I made a simple mechanical load rig to apply cyclic loads to the pile. This rig was developed by Rovere (2004). The rig consists of a steel frame with pulleys, three weight hangers, and a lever...
20 November 2022 5,911 2 View
I would like to recover viruses from ground water to do metagenomic sequencing. From my reading I found the researchers used cellulose nitrate filter membranes. In Egypt the available filter...
16 November 2022 9,838 3 View
our graduation project is about a basket that classifies the garbage so we need to know how to distinguish between the waste
15 November 2021 6,106 4 View
Dear fellow researchers, I have a question concerning LCZ modelling for a masterplan. My case study is on a new city the city is still under construction not completed yet. I want to classify it's...
21 October 2021 6,942 4 View
Hi, I have three questions. I need to arrange the institutions according to the total global citation score (TGCS) in bibliometric analysis. I find that the 1st one was Nova Nordisk AS, and Nova...
15 June 2021 2,178 2 View
Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?
16 July 2024 9,576 1 View
I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery
06 July 2024 246 1 View
We just purchased a multichannel and a single channel E1 Clip tip pipettes. In our first attempt to use them for an experiment they are not dispensing accurately? The rep told me that...
01 July 2024 1,983 0 View
The Evolution and Application of Spinal Anesthesia: Techniqu... What are the primary indications for choosing neuraxial anesthesia over general anesthesia in surgical procedures?
27 June 2024 5,763 1 View
Can we even make changes to CLIP Model architecture such that it can be used as an image generator from prompts ?
16 June 2024 320 0 View
Hello all. I grew ARPE-19 cells in cell culture and infected them with a virus (Varicella-zoster virus). After the virus reached a high infection rate, I harvested everything in the plate and use...
07 May 2024 259 3 View
Suppose austenite finish temperature of Niti alloy wire A is 25 deg Celsius and Niti wire B is 35 deg Celsius. Which one is best recommended for Biliary stent application and why?
29 April 2024 7,585 0 View
Complications associated with paediatric anaesthesia can arise due to various factors, including the child's physiological differences, underlying medical conditions, surgical procedures, and...
09 April 2024 5,900 1 View
Each of the common paediatric surgical procedures mentioned—tonsillectomy, inguinal hernia repair, and appendectomy—poses unique anaesthetic considerations due to differences in patient...
09 April 2024 9,003 1 View
Pharmacological agents used for induction, maintenance, and emergence from anaesthesia in paediatric patients are selected based on factors such as age, weight, medical history, surgical...
09 April 2024 7,682 2 View