would you please inform me if I can detect proteins from serum frozen at -80 C and never thawed
hi
I do not think it's a problem, except for proteins :
1- that have a low stability
2- short half life
3- if you want to work on proteins function
For example, you will not have a problem with Albumin, but you will probably be difficulty with coagulation factors ( such as FVIII or FVII ).
It's completely different from protein to protein.
good luck
what about myokines
what about irisin ?
I have no experience in this. But it's probably a problem with myokines, which is a small cytokine, but I do not think it's an issue with the Irishman. Compare the test for more certainty with a fresh sample and a frozen sample.
you can easily use pre-test before main analysis.
just use the small part of your stocked solution for protein assay with new blood sample from the same species with same conditions .
Thanks a lot for the answers
You should have no problem for an sort of protein and DNA assays .
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