21 July 2018 1 2K Report

My research is on direct injection biodiesel fueled diesel engine using numerical simulation. The software I am using is ANSYS FLUENT and I want the biodiesel chemkin reaction mechanism to help me run my simulation case.

More Peter Otchere's questions See All
How do you obtain a Letter of Access for Citizen Researchers?

Please may I ask if non-salaried Public Contributors conduct data collection interviews or Focus Groups with NHS patients as part of your study? This means that the Public Contributor will either...

29 July 2024 3,085 0 View

Can DEPC-treated water be used as a replacement for OCT when embedding frozen tissue on cryostat?

Hi everyone, I am currently working on RNA extraction on tissue, but unfortunately, I only have large frozen clinical tissue on hand. In order to maximize the RNA integrity, I plan to use...

25 June 2024 7,550 1 View

How to write literature review?

i want to write literature review on the topic " production of 3d PLA filament"

19 June 2024 1,712 2 View

Does Trizol LS yield more and better RNA than normal Trizol?

Hi, I am currently working on RNA extraction on blood serum samples using Trizol. However, the RNA yield is quite bad, on average, only yielded 200ng per 200ul of sample input, and the A260/280...

18 June 2024 6,854 3 View

Unstructured Interview Design?

In my qualitative study, I am trying to avoid the traditional belief that types of qualitative research design are just grounded theory, ethnography, phenomenology, case study, historical, and...

15 June 2024 3,884 1 View

What could dissolve MgO nanoparticles over time?

I have a set-up were I have a bottle with water (DI water, 0.8uS/cm). To that bottle I have added some MgO nanopowder, at a concentration of ~ 0.08mg/ml. It appears that the white powder is...

04 June 2024 9,789 4 View

What strategies are being employed to mitigate the environmental impacts of urbanization, particularly regarding habitat loss and fragmentation?

As urbanization accelerates, the environmental toll of rapid urban growth becomes increasingly apparent. Please pitch your opinion/idea on this question as we explore innovative approaches...

02 June 2024 737 3 View

Histology - mitochondria: Hi RG members. Is anybody aware whether a reliable method for determining mitochondrial density exists for light microscopy?

I would like to look at mitochondrial density in transverse sections of intestine. I do not have access to a TEM. I cannot really determine based on an internet search whether it is feasible to do...

02 June 2024 5,373 2 View

Is my researchGate profile going to be visible on search engine ?

My profile on researchGate is not yet visible on goggle

26 May 2024 5,824 1 View

How do climate change-induced alterations in precipitation patterns impact freshwater availability and quality in different regions globally?

Climate change-induced shifts in precipitation patterns are reshaping the availability and quality of freshwater resources worldwide. Join us in examining the far-reaching implications of these...

19 May 2024 8,764 3 View

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How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

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How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Tangential stiffness option in ansys workbench?

I am not able to find how I can provide tangential stiffness in ansys workbench The normal stiffness option is there. But how I can enter tangential stiffness I have read somewhere tangential...

29 July 2024 2,032 2 View

How to Import Blade geometry from CAD file in ANSYS design modeler?

Hello. I have the geometry of a blade in CAD file (stp) and I want to prepare the blade for meshing with turboGrid. I must import this file into designModeler and then transfer to the...

27 July 2024 356 3 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

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