How we can calculate k constants for a multi-machine power system using PSAT?

Small signal stability analysis of multi-machine power system using PSAT software

06 August 2020 8,195 3 View

Steps to improve cryoEM helical reconstruction resolution using relion?

Hello all, I am trying to do helical reconstruction for my fibrils. I am at 3D classification steps where I can get the resolution (given by relion) at 3.2 A. The actual resolution is worse I...

28 July 2020 6,266 1 View

Does Aristotle have a term and a theory for what the Scholastics have named "ens rationis" as opposed to "ens reale"?

Scholastics have called "entia rationis" those objects, which do not and cannot exist in reality but only within the mind, such as chimaeras, privations (like blindness), negations...

19 July 2020 4,667 22 View

Are there contemporary evaluations of US public health system?

I'm looking for a recent evaluation of the US federal efforts to prevent disease outbreaks, specific to the US public health system. I see some old (15-16 yr old) reports, anyone done anything...

06 June 2020 7,396 1 View

Is there any working method for concentration of influenza virus from water and sediments without the inactivation of the virus?

I have done an experiment to study the persistence of avian influenza in lake water and in lake sediments. Do you know a good concentration method to use without the inactivation of the virus from...

27 January 2020 7,044 3 View

There is any way to know the equivalence between an old genome reference assembly and its new version??

28 November 2019 8,768 4 View

Peptide concentration for stimulating murine CD4+ and CD8+ splenocytes?

I'm trying to figure out a good starting concentration of 15mer peptides to stimulate mouse CD4+ and CD8+ splenocytes for cytokine staining. We are pooling 72 peptides for one of our conditions to...

17 October 2019 6,974 2 View

Can commercial Competitive ELISA Oxytocin kits measure synthetic oxytocin (used in clinical practice)?

Can commercial Competitive ELISA Oxytocin kits measure/pick up synthetic oxytocin (used in clinical practice)?

09 September 2019 6,479 3 View

Is there a statistical techniques to compare multiple subscores of a specific domain?

I performed a neuro-psychological battery consisting of 6 tests that measures different aspects of executive function. Each test has between 3 to 15 subscores. There is no consensus on whether one...

10 June 2019 4,062 3 View

How long does an IAC antibody-coupled NHS column last?

I'm trying to use IAC to purify a protein using an old prepacked NHS column with a potent antibody, that was coupled 2-3 years ago, and getting bad yields (Wide peak over 4 mL at 2 mAU max,...

17 January 2019 5,664 4 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

How to assess monoamine oxidase levels ?

hello everyone i have a research about monoamine oxidase enzyme and i saw a lot of detection method of it including monoamine oxidase activity assay kit but i don't know what is the easiest and...

18 January 2021 3,474 2 View

Two pair of peaks of quinone/hydroquinone redox probe on GCe?

Hello, I recently stumbled upon a voltammogram of HQ/Q in 0.1M NaCl on glassy carbon electrode. I was surprised to see two pairs of peaks present on the graph. As far as I know it is a two...

29 June 2020 1,833 2 View

Can anyone help identify this unknown bacteria?

I'm having extreme difficulty identifying this bacteria... here are the results of some of the tests I've performed. Any insight is greatly appreciated! TTC Motility: strongly motile Gram stain:...

04 March 2020 2,261 6 View

Why when using H2O2 with catechol to estimated ppo enzyme the activity become very high but some time disappear without it?

hi i working on polyphenoloxidase enzyme by using catechol as a substrate but the activity was very week or disappear, and when i added H2O2 with catechol the activity become high . can anyone...

21 January 2020 9,965 3 View

GROMACS - why cannot load the xtc file (without PBC) to VMD?

Dear all, I have a question regarding loading .xtc file (with no periodic boundary conditions) to VMD which I generated before. To this end, I had used the command: trjconv -f md_0_10.xtc -s...

12 January 2020 7,335 5 View

GROMACS - why cannot load the xtc file (without PBC) to VMD?

Dear all, I have a question regarding loading .xtc file (with no periodic boundary conditions) to VMD which I generated before. To this end, I had used the command: trjconv -f md_0_10.xtc -s...

31 December 2019 783 4 View

If you are running western blots on two different tissues do you have to use the same antibodies on both?

I am running western blots on liver and hippocampi from high-fat diet mice and normal diet mice. I would like to investigate whether cytochrome c oxidase (CCOX), a marker of metabolism, is reduced...

10 November 2019 912 4 View

How can we use ECDA(Eicosadienoic acid) to detect amines?

Hello! My coworker have been working with spoilage tests and Ive been helping him. But these days we have a problem about ECDA solution. We couldnt get any colour change when we test it. So I hope...

06 November 2019 9,543 2 View

Does temperature affect the stability of glucose oxidase during immobilization onto working electrode of SPCE for glucose biosensor application?

Dear All, I am working on research related to fabrication of glucose biosensor by using screen printed carbon electrodes (SPCE). I modified the working electrode with nanocomposite and then...

03 November 2019 5,416 3 View