if 1 OD=0.28 mg of glucose standard then how do I find the enzyme unit in millimoles/ml/min. for sample having OD=0.563
Can I use my PCR product (amplified with primers having restriction sites on their ends for both enzymes) directly for restriction with (double digest) two Restrction enzymes ie. Eco RI and Nco...
02 March 2015 471 4 View
I need to know that it has to be scratched from frozen glycerol stock or glycerol stock with bacterial cells can be thawed before use?
31 December 2014 9,906 2 View
I need to prepare L-tyrosine to prepare some modified M9 medium but while preparing it in water it remained turbid and adding HCl while stirring makes it clear but at the same time HCl lowers it...
31 December 2014 9,847 5 View
Sequence is just the same as I ordered or used earlier but the Tm is different. Tm that was ordered by me was 51 and 52 Tm of primers I have received has 69 and 52. Is it possible if the primers...
05 June 2014 1,042 7 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
I'm interested in learning about enzyme usage. Could someone explain the basics of working with enzymes? I understand they're different from standard chemicals, but I'm uncertain about the...
01 August 2024 5,460 5 View
I want to choose a resarch topic regarding enzyme inhibition. So I did my research and found out most of the diseases that originate from enzymes were actually caused by the "deficiency" of...
30 July 2024 2,483 5 View
if you have MRS broth already prepared, what is the manner you will follow for preparing hyper-sweet medium for screening of EPS producer LAB? what the concentration of saccharose or glucose you...
26 July 2024 6,606 0 View
I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...
24 July 2024 9,688 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View
I am working on carbonic anhydrase immobilization into MOFs. I am facing problems with low enzyme loading.. The other issue is that when using p-NPA activity test to detect the activity of the...
20 July 2024 1,440 3 View
These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"
17 July 2024 6,519 3 View