Vipasha Verma

5 Questions 3 Answers 0 Followers

Questions related from Vipasha Verma

Can I double digest directly a PCR product using EcoRI and Nco I without purification? Will EcoRI and Nco I be supported with PCR buffer?

Can I use my PCR product (amplified with primers having restriction sites on their ends for both enzymes) directly for restriction with (double digest) two Restrction enzymes ie. Eco RI and Nco...

03 March 2015 456 4 View

Which is the better procedure to revive a glycerol stock of bacterial culture stored at -80 C?

I need to know that it has to be scratched from frozen glycerol stock or glycerol stock with bacterial cells can be thawed before use?

01 January 2015 9,888 2 View

How can one prepare standard solution of L-tyrosine?

I need to prepare L-tyrosine to prepare some modified M9 medium but while preparing it in water it remained turbid and adding HCl while stirring makes it clear but at the same time HCl lowers it...

01 January 2015 9,826 5 View

Please help me on this

if 1 OD=0.28 mg of glucose standard then how do I find the enzyme unit in millimoles/ml/min. for sample having OD=0.563

09 September 2014 8,248 0 View

Do I need to optimize my PCR conditions for the same set of primers (same sequence), but having different Tm?

Sequence is just the same as I ordered or used earlier but the Tm is different. Tm that was ordered by me was 51 and 52 Tm of primers I have received has 69 and 52. Is it possible if the primers...

06 June 2014 1,026 7 View