How can I label single guided RNA (sgRNA) with Alexa 488 dye and quantum dots (QDs)? I will appreciate any paper recommended or detailed protocols.
Hi Joel, I'm also looking for quantum dots labeled SgRNA, did you have any luck finding commercialized products? Thanks!
what can be the undiscovered benefits of applying telematic technologies for road pavement maintenance, incident reduction and environmental protection?
29 June 2024 7,507 1 View
most of the time when i search fo certain topic i found the human verification respond and nothing change there after
19 March 2024 6,870 0 View
Hello! I have a methodological question and I hope someone can help me. I collected data via an online survey with a sample size of 2600 (N = 2600). Initially, I aimed to examine the effect of...
26 February 2024 6,911 4 View
Hi RG Community I had input all the appropriate files, however, received an Action halted on error! My ORCA generated .gbw QM Output file was not recognized (please see screenshot attached). I...
15 February 2024 2,553 1 View
Typically, it is said that biofilter by their nature allow the settling of bacteria beneficial to the treatment of fishwaste. Fish culture though is known for producing high amounts of ammonia in...
28 January 2024 2,398 3 View
From what I can tell by searching the "Your World in Data" website, eastern Europe seems to have an expected lower than average all-cause mortality rate than the western first world nations, who...
04 January 2024 7,066 0 View
Hello RG Community:), I have a ligand exhibiting penalties between 10-50 within a .str file generated via a CGenFF, the generated .prm file does not exactly match the ligand atoms nor the closest...
25 December 2023 4,364 1 View
Hi I had modelled a heptamer transmembrane protein missing 10-residues within the extracellular loops via MODELLER constraining the known residues as a heptamer (when MODELLER modeled as a monomer...
08 December 2023 4,512 0 View
Hello towards the above topic, I successfully loop-modeled a transmembrane heptamer protein (426-residues per monomer) exhibiting 7-extracellular loops each missing 11-residues via MODELLER via...
03 December 2023 9,528 2 View
Need a simulation tool to generate synthetic data Specialized engine simulation software (for control and diagnosis) how and what to do 🙏?
16 November 2023 4,128 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View